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Cloud of Unkowing Abstract

Page history last edited by mobius@... 11 years, 3 months ago
Spiritual Tourism • entheogens •Kali • Sensory Motor Ecstasy•Secret substances and ritual manifestationsAmanita muscaria,placental sac of spiderlings• Daimonic Self Organization•mythopoiesis•


Abstract: The author presents a case of self organizing daimonic mythopoiesis. After an encounter with Kali, fungal and root cortical cells compete for monosaccharides, generated from plant-derived sucrose. Therefore, the kinetic properties of the monosaccharide uptake systems are decisive for the monosaccharide yield of each partner. For the functional characterization of a hexose transporter (AmMst1) of the ectomycorrhizal fungus Amanita muscaria, the entire cDNA was expressed in a Saccharomyces cerevisiae strain unable to take up hexoses. Uptake experiments with 14C-labelled monosaccharides resulted in KM values of 0.46 mM for glucose and 4.20 mM for fructose, revealing a strong preference of AmMst1 for glucose as substrate. Glucose uptake by AmMst1 was strongly favoured even in the presence of a large excess of fructose. Comparable affinities of AmMst1 for glucose, 3-O-methyl glucose and mannose were obtained. In contrast, AmMst1 imported galactose with a much lower efficiency, revealing that this transporter distinguishes pyranoses by steric hindrance at the C-4 position. While yeast contains numerous hexose transporter genes, the AmMst1 gene seems to be the main, if not the only, hexose transporter that is expressed in A. muscaria, as concluded from the comparison of hexose import properties of A. muscaria protoplasts and AmMst1 expressed in yeast.

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